Two-stage Nicking Enzyme Signal Amplification Combined with DNAzyme Amplification for the Detection of Bone Morphogenetic Protein 6 mRNA
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چکیده
منابع مشابه
Nicking enzyme based homogeneous aptasensors for amplification detection of protein.
A simple and highly sensitive homogeneous aptasensor is developed, which relies on nicking enzyme. The sensitivity of this newly proposed aptasensor is about three orders of magnitude higher than that of traditional homogeneous aptasensors. Furthermore, it is capable of detecting target protein in real samples.
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An isothermal and sensitive fluorescence assay for protein detection using aptamer-protein-aptamer conjugates based on nicking enzyme amplification has been developed, which was combined with magnetic microparticles separation, making this assay suitable for protein detection in biological samples.
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synthesized from Takara Biotechnology Co. Ltd. (Dalian, China). Recombinant human PDGF-BB and PDGF-AB were purchased from Bowling Vaccine & Pharmaceutical Inc. (San Francisco, CA). Bovine serum albumin (BSA), human serum albumin (HSA), human immunoglobulin G (IgG), thrombin, bovine serum were obtained from Dingguo Biotechnology Co. Ltd. (Beijing, China). Escherichia coli Exonuclease I (Exo I) w...
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An ultrasensitive fluorescence detection method for DNA based on nicking endonuclease (NEase) and target recycles assisted with CdTe quantum dots (QDs) is reported. In the detection system, when the target DNA is present, it hybridizes with a linker strand to from a duplex, in which the NEase recognizes specific nucleotide sequences and cleaves the linker strand. After nicking, the fragments of...
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A simple, highly sensitive and enzyme-free DNAzyme sensor based on target-catalyzed hairpin assembly is developed, which permits detection of 0.1 pM target DNA. Furthermore, this DNAzyme sensor is capable of detecting target DNA in real samples because of its high selectivity.
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ژورنال
عنوان ژورنال: Analytical Sciences
سال: 2014
ISSN: 0910-6340,1348-2246
DOI: 10.2116/analsci.30.1039